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69892

Sigma-Aldrich

MES monohydrate

BioXtra, ≥99.0% (T)

Synonym(s):
2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate
Empirical Formula (Hill Notation):
C6H13NO4S · H2O
CAS Number:
Molecular Weight:
213.25
Beilstein:
6350956
EC Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioXtra

assay

≥99.0% (T)

form

powder or crystals

useful pH range

5.5-6.7

pKa (25 °C)

6.1

mp

>300 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

application(s)

diagnostic assay manufacturing

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Application

MES Monohydrate has been used:
  • To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
  • As a buffering agent to stabilize enzymatic solutions
  • As a component of PAGE running buffer

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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