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68268

Sigma-Aldrich

Imidazole buffer Solution

BioUltra, 1 M in H2O

Synonym(s):
Imidazole solution, Glyoxaline solution
Empirical Formula (Hill Notation):
C3H4N2
CAS Number:
Molecular Weight:
68.08
Beilstein:
103853
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioUltra

Quality Level

form

liquid

concentration

1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

9.5±0.5

density

1.01 g/mL at 20 °C

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

Related Categories

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Application

Imidazole buffer solution has been used as a component:
  • of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
  • of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
  • of imidazole wash and elution buffer for protein purification

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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