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Sigma-Aldrich

iTRAQ® Reagent Application Kit - Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.24

shipped in

dry ice

storage temp.

−20°C

General description

Contains sufficient reaction solutions, test mixture and iTRAQ® Reagents 114, 115, 116 and 117 (for 25 μg protein) to label 10 duplex, 6 three-plex or 5 four-plex experiments.

Application

This iTRAQ application kit for protein provides iTRAQ reagents and protocol to label protein prior to digestion. This iChemistry kit can be used in workflows that include SDS-PAGE separation and in-gel protein digestion, followed by LC/MS/MS analysis. Trypsin is sold separately.

Analysis Note

To view the Protocol for the iTRAQ Reagents 4-plex Application Kit - Protein, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

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Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oded Kleifeld et al.
Nature protocols, 6(10), 1578-1611 (2011-10-01)
Analysis of the sequence and nature of protein N termini has many applications. Defining the termini of proteins for proteome annotation in the Human Proteome Project is of increasing importance. Terminomics analysis of protease cleavage sites in degradomics for substrate
Yiping Huang et al.
Cell cycle (Georgetown, Tex.), 11(12), 2367-2379 (2012-06-08)
Cisplatin chemoresistance is a clinical problem that leads to treatment failure in various human epithelial cancers. Members of tumor protein (TP) p53 family play various critical roles in the multiple molecular mechanisms underlying the chemoresistance of tumor cells. However, the

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