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30434

Sigma-Aldrich

Dansylhydrazine

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

5-(Dimethylamino)-1-naphthalenesulfonic hydrazide, 5-(Dimethylamino)naphthalene-1-sulfono­hydra­zide, Dns-Hz

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About This Item

Linear Formula:
(CH3)2NC10H6SO2NHNH2
CAS Number:
33008-06-9
Molecular Weight:
265.33
Beilstein/REAXYS Number:
2868662
EC Number:
251-337-7
MDL number:
MFCD00003986
UNSPSC Code:
12352103
PubChem Substance ID:
329753512
NACRES:
NA.32

product line

BioReagent

assay

≥90% (HPLC)

mp

126-130 °C (lit.)

solubility

ethanol: soluble

fluorescence

λex 340 nm; λem 525 nm in ethanol

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CN(C)c1cccc2c(cccc12)S(=O)(=O)NN

InChI

1S/C12H15N3O2S/c1-15(2)11-7-3-6-10-9(11)5-4-8-12(10)18(16,17)14-13/h3-8,14H,13H2,1-2H3

InChI key

KPQYDVAFRDWIBW-UHFFFAOYSA-N

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Application

Dansylhydrazine (DH) is widely used as a fluorometric reagent for carbonyl compounds. Dansylated carbonyl molecules can be separated and identified by analytical techniques.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Fluorescent marker for carbonyl compounds

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Analytical derivatization (AD) increases the sensitivity of analysis by one to three orders of magnitude, stabilizes labile analytes and converts them into readily extractable products. Using a variant of this technique, we applied solid phase analytical derivatization (SPAD) to fully
1-Dimethylaminonaphthalene-5-sulfonyl hydrazine (dansyl hydrazine): a fluorometric reagent for carbonyl compounds.
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J M Anderson
Analytical biochemistry, 152(1), 146-153 (1986-01-01)
Methods for the determination of carbonyl compounds of biological origin by high-performance liquid chromatography were improved by the use of new fluorescent derivatizing agents. Eight fluorescent hydrazides were either synthesized or obtained commercially and compared to dansyl hydrazine (1-dimethylaminonaphthalene-5-sulfonylohydrazide). Four
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