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14479

Sigma-Aldrich

Phalloidin-Atto 490LS

suitable for fluorescence

Synonym(s):

Atto 490LS-Phalloidin

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

assay

≥75.0% (HPLC)

Quality Level

mol wt

Mw 1466 g/mol

manufacturer/tradename

ATTO-TEC GmbH

λ

in acetonitrile: water (1:1)

UV absorption

λ: 495-501 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1.
Phalloidin, a bicyclic heptapeptide, is a very strong binding reagent to actin. Fluorescent labeled phalloidin has become a useful tool to investigate the distribution of F-actin within the cytoskeleton of cells by fluorescence microscopy.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Xiaomeng Zhang et al.
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