MilliporeSigma
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108319

SAFC

TRIS hydrochloride

EMPROVE® EVOLVE

Synonym(s):
TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, Trizma hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
MDL number:

Quality Level

biological source

synthetic

description

Manufactured under appropriate controls for use as a raw material in BioProcessing

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested
Microbial, tested
Trace Metals, tested

useful pH range

7.0-9.0

pKa (25 °C)

8.1

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, contains (tested)
DNase, contains (tested)
NICKase, contains (tested)
Protease, contains (tested)
RNase, contains (tested)

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Application

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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