108319

TRIS hydrochloride

EMPROVE^® EVOLVE

• Synonym(s): TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, Trizma hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• MDL number: MFCD00012590

Properties

• Quality Level: 400
• biological source: synthetic
• description: Manufactured under appropriate controls for use as a raw material in BioProcessing
• product line: EMPROVE^® EVOLVE
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin, tested; Microbial, tested; Trace Metals, tested
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, contains (tested); DNase, contains (tested); NICKase, contains (tested); Protease, contains (tested); RNase, contains (tested)
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable