108319

TRIS hydrochloride

EMPROVE^® EVOLVE

• Synonym(s): Trizma^® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• MDL number: MFCD00012590

Properties

• biological source: synthetic
• Quality Level: 400
• description: Manufactured under appropriate controls for use as a raw material in BioProcessing
• product line: EMPROVE^® EVOLVE
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin, tested; Microbial, tested; Trace Metals, tested
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, contains (tested); DNase, contains (tested); NICKase, contains (tested); Protease, contains (tested); RNase, contains (tested)
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable