60220

Potassium phosphate monobasic

buffer substance, anhydrous, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.5-100.5%

• Synonym(s): Monopotassium phosphate, Potassium dihydrogen phosphate, prim.-Potassium phosphate
• Linear Formula: KH₂PO₄
• CAS Number: 7778-77-0
• Molecular Weight: 136.09
• EC Number: 231-913-4
• MDL number: MFCD00011401
• UNSPSC Code: 12352300
• PubChem Substance ID: 329758828
• NACRES: NA.21

Properties

• grade: ACS reagent; anhydrous; puriss. p.a.
• Quality Level: 100
• agency: reag. ISO; reag. Ph. Eur.
• assay: 99.5-100.5%
• form: crystalline
• impurities: ≤0.001% total nitrogen (N)
• loss: ≤0.2% loss on drying, 105 °C
• pH: 4.2-4.5 (20 °C, 5%)
• pKa (25 °C): (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
• mp: 252.6 °C (lit.)
• solubility: water: 0.5 g/10 mL, clear, colorless to light yellow
• density: 2.338 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤30 mg/kg
• cation traces: As: ≤0.5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤15 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg; heavy metals: ≤0.001% (by ICP)
• SMILES string: [K+].OP(O)([O-])=O
• InChI: 1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1
• InChI key: GNSKLFRGEWLPPA-UHFFFAOYSA-M

Description

General description

Potassium phosphate is widely used in buffers to prepare biological samples and examine biological interactions.

Application

Potassium phosphate monobasic has been used:

• to prepare the working luminol solution to study the prooxidant activity in the chemiluminescent system luminol–H₂O₂
• in phosphate buffer saline (PBS) to culture embryonic stem cells (ESCs) and generate embryoid bodies
• in PBS to prepare polydimethylsiloxane (PDMS) substrate

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable