certified by the Biological Stain Commission
Dye content, ≥75%
120 °C (dec.) (lit.)
chloroform/ethanol (1:1): 1 mg/mL
diagnostic assay manufacturing
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
The Oil Red O is only minimally soluble in the solvent. Solubility is typically decreased by diluting the stock Oil Red O solutions in water before use. The Oil Red O then moves from the solution to the lipid - where there is greater solubility.
Yes, there are several. Working solutions prepared without dextrin typically have shorter stability dating. The method of the Biological Stain Commission is listed in: Penny, DP et al, Analysis and testing of biological stains - The Biological Stain Commission Procedures. Biotech. Histochem. 77(5&6), 237-275, (2002). The Churukian method is also listed in Bancroft, J and Gamble, M; Theory and Practice of Histological Techniques, Churchill Livingstone, New York 2002 pages 207-208.
No. The solvents typically used in paraffin processing will dissolve away most of the fats and lipids. Frozen sections are the preferred tissue specimen.
Yes, although the cultured cells must first be fixed before staining. Preferred fixatives would include aqueous fixatives such as 10% neutral buffered formalin. Avoid fixatives such as ethanol or acetone. After fixation and rinsing, the cells may be stained with the basic procedures used for staining tissues attached to glass slides
Each lot of Product O0625 has been certified for use in histological applications by the Biological Stain Commission. It is not specifically use-tested for any electrophoresis application. We offer a different grade of Oil Red O (Product No. O9755) which has been tested for suitability in electrophoresis; each lot of O9755 has been tested and found suitable for staining lipoproteins that have been separated by cellulose acetate electrophoresis.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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