All Photos(4)

E9884

Ethylenediaminetetraacetic acid

ACS reagent, 99.4-100.6%, powder

All Photos(4)

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

Linear Formula:

(HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂

CAS Number:

60-00-4

Molecular Weight:

292.24

Beilstein:

1716295

EC Number:

200-449-4

MDL number:

MFCD00003541

eCl@ss:

39030907

PubChem Substance ID:

24894705

NACRES:

NA.77

Quality Level

200

grade

ACS reagent

assay

99.4-100.6%

form

powder

impurities

≤0.005% Insol. dil. NH₄OH
≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

ign. residue

≤0.2%

mp

250 °C (dec.) (lit.)

solubility

water: soluble 0.4 g/L at 20 °C

cation traces

Ca: ≤0.001%
Fe: ≤0.005%
Mg: ≤5 ppm
heavy metals (as Pb): ≤0.001%

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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Related Categories

Chelating & Reducing Agents

General description

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.

Application

Ethylenediaminetetraacetic acid has been used:

as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
for investigating the effect of papain enzyme on wound debridement and other skin issues
for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis

Packaging

100, 500 g in poly bottle

1 kg in poly bottle

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany. It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.

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Pictograms

GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)