MilliporeSigma
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88597

Millipore

Hydrogen peroxide solution

3%, suitable for microbiology

Synonym(s):
Catalase Test
Empirical Formula (Hill Notation):
H2O2
CAS Number:
Molecular Weight:
34.01
Beilstein:
3587191
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Quality Level

Agency

according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017

vapor pressure

23.3 mmHg ( 30 °C)

assay

2.8-3.2% (redox titration)

form

solution

shelf life

limited shelf life, expiry date on the label

concentration

1-5%

technique(s)

microbe id | specific enzyme detection: suitable

mp

0.0 °C

density

1.000 g/cm3

application(s)

clinical testing
environmental
food and beverages
veterinary

microbiology

suitability

Pneumococcus spp.
Staphylococcus spp.
bacteria

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.

Application

Hydrogen peroxide solution has been used:
  • to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
  • for the induction of apoptosis in cultured human microvascular endothelial cells
  • as a substrate for superoxide dismutase

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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