All Photos(1)

88597

Millipore

Hydrogen peroxide solution

3%, suitable for microbiology

Synonym(s):
Catalase Test
Empirical Formula (Hill Notation):
H2O2
CAS Number:
Molecular Weight:
34.01
Beilstein:
3587191
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Quality Level

agency

according to GB 4789.30-2016
according to ISO 10272-1:2017
according to ISO 10272-2:2017
according to ISO 11290-1:2017
according to ISO 11290-2:2017

vapor pressure

23.3 mmHg ( 30 °C)

assay

2.8-3.2% (redox titration)

shelf life

limited shelf life, expiry date on the label

concentration

1-5%

technique(s)

microbe id | specific enzyme detection: suitable

mp

0.0 °C

density

1.000 g/cm3

application(s)

clinical testing
environmental
food and beverages
veterinary

microbiology

suitability

Pneumococcus spp.
Staphylococcus spp.
bacteria

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.

Application

Hydrogen peroxide solution has been used:
  • to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
  • for the induction of apoptosis in cultured human microvascular endothelial cells
  • as a substrate for superoxide dismutase

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

More documents

Quotes and Ordering

Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
Choi S and Love PE
Bio-protocol, 8(1) (2018)
Superoxide is the critical driver of DOPAL autoxidation, lysyl adduct formation, and crosslinking of alpha-synuclein
Werner-Allen JW, et al.
Biochemical and Biophysical Research Communications, 487(2), 281-286 (2017)
Bach1 induces endothelial cell apoptosis and cell-cycle arrest through ROS generation
Wang X, et al.
Oxidative Medicine and Cellular Longevity, 2016(1) (2016)
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice
Koen van der Maaden et al.
Pharmaceutical research, 31(7), 1846-1854 (2014-01-29)
The aim of the study was to develop a cheap and fast method to produce hollow microneedles and an applicator for injecting vaccines into the skin at a pre-defined depth and test the applicability of the system for dermal polio

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