Poly(vinylpolypyrrolidone) is a crosslinked homopolymer form of purified vinylpyrrolidone. It is hygroscopic in nature. It has been used widely in plant hormone purification. It binds with phenolic compounds by hydrogen bonding.
It was used as lysis buffer in measuring DNA concentration. It was also used in detection of recombinant proteins from leaf extracts using Surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS).
Features and Benefits
Complexes with phenolics and alkaloids for their removal from plant samples, thus preventing their modification of proteins and any interference they may cause in spectrophotometric determinations of protein content. This is also reported to improve stability of enzymes.