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MRS Agar, Vegitone

suitable for microbiology, NutriSelect® Plus

Synonym(s):

DeMan, Rogosa and Sharpe Agar, Vegitone, Vegitone MRS Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
ammonium citrate, 2.0 g/L
bromocresol green, 0.04 g/L
captan, 0.004 g/L
dextrose, 20.0 g/L
dipotassium phosphate, 2.0 g/L
magnesium sulfate, 0.1 g/L
manganese sulfate, 0.05 g/L
2-phenylethyl alcohol, 3.0 g/L
proteose peptone (vegetable), 10.0 g/L
sodium acetate, 5.0 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

selective and differential for Lactobacillus spp.
selective by low pH for Bifidobacterium spp.

General description

This MRS Agar is free of animal derived material. It is recommended for the isolation and cultivation of Lactobacillus species.

Application

MRS Agar, Vegitone has been used for culturing yogurt to analyze lactic acid bacteria (LAB). It has also been used in culturing of Lactobacillus reuteri colonies.

Preparation Note

Suspend 62.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Cat. No. P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG5976
BCCF0772
BCCC1959
BCBZ1789
BCBX5261

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He Ni et al.
Heliyon, 4(10), e00875-e00875 (2018-11-06)
In this study aqueous extracts from salal berry (SB) and blackcurrant pomace (BCP) were used to reformulate yogurt and the anti-diabetic properties of the beverage were investigated during 4 weeks of cold storage at 4 °C. Results indicated that α-amylase
Ferdinand Sedlmayer et al.
Nature communications, 9(1), 1822-1822 (2018-05-10)
Quorum sensing is a promising target for next-generation anti-infectives designed to address evolving bacterial drug resistance. The autoinducer-2 (AI-2) is a key quorum-sensing signal molecule which regulates bacterial group behaviors and is recognized by many Gram-negative and Gram-positive bacteria. Here

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