0018-FP

Neu5Gcα-C3-PAA-fluo

• UNSPSC Code: 12352201
• NACRES: NA.25

Properties

• carrier: PAA carrier
• label: FITC labeled
• product line: FP-Series
• assay: 95% (H-NMR)
• form: lyophilized powder
• mol wt: ~20,000 Da
• manufacturer/tradename: GlycoNZ
• extent of labeling: 1% Biotin per 20 mole % Poly[N-(2-hydroxyethyl)acrylamide-co-N-acrylamide] ligand density
• technique(s): flow cytometry: suitable
• probe type: monosaccharide
• probe presentation: polymeric
• storage temp.: −20°C

Description

General description

FP-Series: Fluorescein-Labeled Polymeric Probes
These glycoconjugate probes are designed to reveal lectins, including selectins, directly on cell membranes, organelles, and in cytoplasm. The low fluorescein content ensures that probe solubility in water is not affected, but still allows reliable probe detection. The affinity of the probes is 10² to 10⁵ times higher than that of corresponding free sugars.

PAA is poly[N-(2-hydroxyethyl)acrylamide], a flexible polymer chain which serves as an additional spacer. Used as a molecular weight carrier, PAA demonstrates an absence of non-specific interaction with cells, and is stable to chemical and proteolytic action.

Application

Glycoconjugates are used to study the functions of carbohydrates, carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.

Preparation Note

Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% (HPLC and ¹H-NMR)

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable