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ABTS-RO

Roche

ABTS Tablets

tablet, green

Synonym(s):

ABTS

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About This Item

UNSPSC Code:
12352200

form

tablet

packaging

pkg of 20 tablets (11112422001[50mg])
pkg of 20 tablets (11204521001[5mg])

manufacturer/tradename

Roche

color

green

solubility

water: soluble

λmax

414 nm

shipped in

wet ice

storage temp.

2-8°C

General description

ABTS Tablets are used as substrates in enzyme immunoassays with horseradish peroxidase as a marker enzyme. It is widely used in spectrophotometric methods to measure antioxidant activities of food and beverages.

Application

ABTS Tablets are the ideal substrate for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.

Features and Benefits

  • ABTS is soluble in aqueous and organic solvents
  • ABTS method is suitable for various pH levels
  • Samples react with ABTS quickly and reach stability within 30 minutes

Packaging

  • 11204521001 (5 mg/tablet; each tablet is for 5 mL substrate solution)
  • 11112422001(50 mg/tablet; each tablet is for 50 mL substrate solution)

Preparation Note

Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of the ABTS buffer solution. This solution has a light-green color. In microtiter plates, it is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.

Analysis Note

Evaluation: photometric (405nm)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ABTS is a trademark of Roche

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Haralg Seitz and Sarah Schumacher
Molecular Diagnostics null
R Re et al.
Free radical biology & medicine, 26(9-10), 1231-1237 (1999-06-25)
A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS*+) is generated by oxidation

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