2GFRMKB
Roche
KAPA2G Fast ReadyMix
suitable for PCR, 2 ×, with dye
Synonym(s):
PCR, mastermix
About This Item
Recommended Products
Quality Level
usage
sufficient for 100 reactions
sufficient for 2000 reactions
sufficient for 500 reactions
shelf life
≤18 mo.
feature
Fast PCR
dNTPs included
hotstart: no
packaging
kit of 1.25 mL (100 x 25 μL rxn; KK5101)
kit of 25 mL (KK5103)
kit of 6.25 mL (500 x 25 μL rxn; KK5102)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
General description
KAPA2G Fast ReadyMix™ PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
Application
- Nested PCR (nPCR)
- Routine PCR
- Fast PCR
- Genotyping
Biochem/physiol Actions
Features and Benefits
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance:
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes:
- KAPA2G Fast ReadyMix PCR Kits contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primerannealing across a wide range of primer lengths, GC contents, and melting temperatures.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- KAPA2G Fast ReadyMix contains 1.5 mM MgCl2 at 1X.KAPA2G Fast ReadyMix includes two inert tracking dyes to allow direct loading onto agarose gels.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Preparation Note
Other Notes
Legal Information
Kit Components Only
- KAPA2G Fast HotStart® DNA Polymerase 0.5 U per 25 µL reaction
- MgCl2 1.5mM at 1X
- Stabilizers
- dNTP Mix 0.2 mM of each dNTP at 1X
signalword
Warning
hcodes
Hazard Classifications
STOT SE 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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