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usage
sufficient for 200 reactions
sufficient for 500 reactions
Quality Level
shelf life
≤18 mo.
feature
Fast PCR
Multiplex PCR
dNTPs included: no
hotstart: no
packaging
pkg of 100 U (KK5020)
pkg of 250 U (KK5021)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
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General description
Application
- routine polymerase chain reaction (PCR)
- multiplex PCR
- rapid photo PCR samples
- Fast PCR
- genotyping
Biochem/physiol Actions
Features and Benefits
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes :
- KAPA2G Fast PCR Kits contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
- Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Preparation Note
Other Notes
Kit Components Only
- KAPA2G Fast DNA Polymerase 5 U/µL
- 5X KAPA2G Buffer A
- KAPA MgCl2 25 mM
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
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Articles
Fast PCR is PCR in which total reaction time is decreased by reducing the duration of one or more of the steps during each cycle of a PCR. Using the KAPA2G Fast PCR Kit, PCR time can be reduced by 20%–70% by simply reducing the extension time in each cycle. Additional timesaving may be achieved by optimizing other cycling parameters (e.g. denaturation and/or annealing time) for your specific assay and thermal cycler.
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