11162306001

ATP-γ-S

=90% (HPLC), solution, pkg of 200 μL (20 μmol)

• Synonym(s): adenosine-5′-o-(3-thio-triphosphate)
• UNSPSC Code: 12352204

Properties

• Quality Level: 100
• description: C₁₀H₁₆N₅O₁₂P₃SLi (Formula); Lithium salt
• assay: 90% (HPLC)
• form: solution
• mol wt: 523.2
• packaging: pkg of 200 μL (20 μmol)
• manufacturer/tradename: Roche
• λmax: 260 nm at 100 mmol/L
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Adenosine 5′-O-(3-thiotriphosphate), lithium salt, solution.

Application

ATP-γ-S has been used:

• to treat THP-1 macrophage cell lines for testing if endoplasmic reticulum (ER) stress causes the production of interleukin (IL)-1β
• in amide hydrogen exchange (HX) experiments coupled to mass spectrometry and study the cyclomarin A (CymA)-induced structural changes in N_Mtb-ClpC
• as a supplement in CMG-binding buffer for gel-based DNA unwinding assays

Biochem/physiol Actions

Adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S) is a substrate and inhibitor of ATP-dependent enzyme systems. It is hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. ATP-γ-S serves as a damage-associated molecular pattern molecule (DAMP) and NLR family pyrin domain containing 3 (NLRP3) inflammasome activator. ATP-γ-S may serve as a substrate for RNA-stimulated nucleotide hydrolysis and RNA unwinding activities of eukaryotic initiation factor-4A (eIF4A).

Quality

Contaminants: ≤10% ADP (HPLC)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available