10736619001

Ascorbate Oxidase Spatula

from Cucurbita species

• Synonym(s): ascorbate oxidase
• Enzyme Commission number: 1.10.3.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: plant (Cucurbita species)
• Quality Level: 100
• form: solid
• specific activity: 17 units
• packaging: pkg of 25 ea
• manufacturer/tradename: Roche
• storage condition: protect from light
• concentration: ≥0.1-1.0 % (w/w)
• technique(s): activity assay: suitable
• color: light gray
• optimum pH: ~6.0
• pH range: 5.6-7.0
• solubility: water: partially soluble
• suitability: suitable for HPLC
• NCBI accession no.: BAA09528
• UniProt accession no.: P24792
• application(s): food and beverages; life science and biopharma
• storage temp.: 2-8°C

Description

General description

L-ascorbate:oxygen oxidoreductase

Specificity

Specific activity: One spatula contains approximately 17 U ascorbate oxidase at +25°C with L-ascorbate as the substrate.

Application

The spatula serves to remove ascorbic acid from aqueous solutions in which the presence of ascorbic acid (ascorbate, vitamin C) can interfere with the investigation of the sample material, for example, the detection of blood (erythrocytes) in urine or the determination of oxalic acid in urine during research studies.
The ascorbic acid to be removed should not exceed a concentration of 10 μg/ml. For concentrations in the range of 50 to 100 μg/ml, stir for 15 minutes. For higher concentrations, the solution should be appropriately diluted.

Physical form

Spatulas

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash