04743784001

GC-RICH PCR System, dNTPack

suitable for PCR, hotstart: no, Difficult Templates/Specialty Enzymes PCR

• Synonym(s): GC-rich PCR
• UNSPSC Code: 41106300
• NACRES: NA.21

Properties

• usage: sufficient for ≤50 reactions
• feature: Difficult Templates/Specialty Enzymes PCR; dNTPs included; hotstart: no
• packaging: pkg of 100 U
• manufacturer/tradename: Roche
• parameter: 72 °C optimum reaction temp.
• technique(s): PCR: suitable
• input: purified DNA
• shipped in: dry ice
• storage temp.: −20°C (−15°C to −25°C)

Description

General description

The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.

Application

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.

Features and Benefits

• Ease access to difficult templates:-

including GC-rich targets and repetitive sequences.

• Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
• Amplify DNA fragments up to 5 kb.
• Cost-effective:-

use the convenient premixed solution of PCR grade NTPs.

Packaging

1 kit containing 6 components

Quality

The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.

Unit Definition

Volume Activity: 2 U/μl

Preparation Note

Working concentration: The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

• GC-RICH Enzyme Mix, in storage buffer
• GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl₂ and DMSO 5x concentrated
• GC-RICH Resolution Solution 5 M
• MgCl₂ Stock Solution 25 mM
• Water, PCR Grade
• PCR Nucleotide Mix

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash