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UFC8050

Millipore

Amicon® Ultra-4 Centrifugal Filter Unit

Ultracel-50 regenerated cellulose membrane, 4 mL sample volume

Synonym(s):
Centrifugal concentrator, 50 kDa MWCO, 4 mL sample volume
eCl@ss:
36100101
NACRES:
NB.24

Quality Level

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

4 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

124 mm (4.88 in.)

diam.

17.3 mm

filtration area

3 cm2

pore size

50 kDa NMWCO

shipped in

ambient

General description

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 50,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example,BSA (67 kDa) at 1 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

  • Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 50 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipette sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

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Certificate of Quality

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