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Key Documents

UFC703008

Millipore

Centricon® Plus Centrifugal Filter

MWCO 30 kDa, sample volume 15-70 mL, regenerated cellulose membrane

Synonym(s):

Centrifuge concentrator

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

polypropylene cap
polypropylene cup (for concentrate/retentate)
polypropylene cup (for filtrate collection)
polypropylene cup (for sample filter)
regenerated cellulose membrane

Quality Level

sterility

non-sterile

product line

Amicon®

feature

holdup volume 75 μL

manufacturer/tradename

Amicon® Ultra
Centricon®

parameter

15-70 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

121 mm

diam.

6 cm

filtration area

19 cm2

pore size

30 kDa MWCO

application(s)

sample preparation

shipped in

ambient

General description

Filter Type: Ultrafiltration
The Centricon Plus-70 centrifugal filter is a disposable, single-use device designed for rapid processing of aqueous biological solutions in volumes ranging from 15 to 70 mL. It is compatible with swinging-bucket centrifuges only. The Centricon Plus-70 device can concentrate most 70 mL solutions down to 350 μL in just 25 minutes (some solutions may take slightly longer).


•90% typical recovery
•50X to 200X concentration
•Low hold-up volume
•Polypropylene housing minimizes binding
•Deadstop prevents spinning to dryness
•Ready to use. Nothing to assemble


Applications:
•Concentrating and desalting chromatography column eluates
•Concentrating monoclonal antibodies
•Concentrating proteins or viruses from culture supernatants
•Clarifying tissue homogenates and cell lysates
•Buffer exchange or diafiltration

Application

30 kDa cutoff, 15 mL to 70 mL volume

Features and Benefits

Minimum Final Concentrate Volume: 350 µL

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
CENTRICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Large-volume centrifugal filter devices are a convenient alternative to stirred cell ultrafiltration devices for purifying proteins from large volumes of solution.

This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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