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SCM308

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3dGRO® Lung Organoid Maturation Medium

Serum-free ready-to-use media used to differentiate lung bud organoids (LBOs) into mature branching lung organoids.

Synonym(s):

3dGRO Maturation Medium, Lung Organoid Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

General description

The lung epithelium undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi, bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include: anterior/posterior and dorsal/ventral patterning, lung specification, lung budding, branching morphogenesis, and maturation.

The 3dGRO Lung Organoid Maturation Medium is a chemically defined/serum-free ready-to-use media used to differentiate lung bud organoids (LBOs) into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. Large numbers of mature lung organoids may be generated that express appropriate markers indicative of multiple cell types found in the mature lung and airways including: SFTPB and SFTPC, MUC5AC, EpCAM, Sox9, Nkx2.1, acetyl-a-tubulin and Vimentin. Additionally, the mature lung organoids express ACE2 and TMPRSS2 for SARS-CoV2 viral research. The 3dGRO Lung Organoid Maturation Medium is a component of the 3dGRO Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures.The 3dGRO® Human Lung Organoid Culture System includes (all sold separately):

  • Definitive Endoderm Induction Medium (SCM302)
  • Anterior Foregut Endoderm Induction Medium I (SCM305)
  • Anterior Foregut Endoderm Induction Medium II (SCM306)
  • 3dGRO® Lung Organoid Branching Medium (SCM307)
  • 3dGRO® Lung Organoid Maturation Medium (SCM308)

Human iPSC Derived AFE Progenitors (SCC301)

Storage and Stability

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

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Articles

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

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