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SCC325

Sigma-Aldrich

3dGRO Colon Intestinal Organoids, Age 55 (Prep 89-C)

Cryopreserved patient derived gastrointestinal organoids (PDOs)

technique(s)

cell culture | stem cell: suitable

Related Categories

General description

Patient derived organoids (PDOs) are novel in vitro 3D cell models that preserve original tissue physiology and molecular pathology, thus representing a clinically-relevant alternative to traditional 2D cell lines and an effective tool to refine and reduce animal models. PDOs can be derived from adult patient biopsies or resected tissues containing native LGR5+ stem cell populations and cultured within an ECM-rich substrate using specialized organoid media such as L-WRN conditioned media. Adult tissue derived organoids are phenotypically and genetically stable in long term culture, presenting more mature phenotypes compared to iPSC-derived organoids. Importantly, PDOs have shown to be able to predict patient clinical responses to chemotherapeutics.
We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.
Cell Line Characteristics
  • Sex: Male
  • Age: 55 Years
  • Organ: Colon
  • Disease: Normal

Cell Line Origin

Human, organoid

Packaging

≥1500 viable organoids/vial

Quality

  • Viability: ≥1500 viable organoids/vial
  • Organoid Growth: Pass
  • Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
  • Mycoplasma Contamination: Negative
  • STR Profile: Pass

Storage and Stability

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Legal Information

3dGRO organoids were derived utilizing HUB Organoid Technology
3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. The purchaser of this product shall agree to HUB′s terms of use, which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this Product to purchaser.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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SDS

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