MilliporeSigma
All Photos(1)

SCC301

Sigma-Aldrich

Human iPSC Derived AFE Progenitors

Cryopreserved human iPSC derived anterior foregut endoderm (AFE) progenitor cells that express Sox2, Pax9 and EpCAM.

technique(s)

cell culture | stem cell: suitable

application(s)

cell analysis

Related Categories

General description

The anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the lung, esophagus, salivary glands, thymus, parathyroid and thyroid. Our cryopreserved anterior foregut endoderm (AFE) progenitor cells were derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550) in chemically defined/serum-free culture conditions. The AFE cells are double positive for Sox2 (A,C; Sigma AB5603A4; 1:50), Pax9 (B, C; Abcam ab28538; 1:200) and approximately 20-30% are double positive for EpCAM (D, F; Sigma MAB4444; 1:500) and Pax9 (E, F).

The human iPSC derived AFE progenitors are a component of the 3dGRO Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the <italic>in vivo</italic> branching airway and early alveolar structures. The 3dGRO Human Lung Organoid Culture System includes (all sold separately):

  • Definitive Endoderm Induction Medium (SCM302)
  • Anterior Foregut Endoderm Induction Medium I (SCM305)
  • Anterior Foregut Endoderm Induction Medium II (SCM306)
  • 3dGRO Lung Organoid Branching Medium (SCM307)
  • 3dGRO Lung Organoid Maturation Medium (SCM308)
  • Human iPSC Derived AFE Progenitors (SCC301)



Research Category:
  • Cell Culture
  • Stem Cell Research

Cell Line Origin

Human, Organoid

Packaging

3X106 cell/vial

Storage and Stability

Human iPSC Derived AFE Progenitors should be stored in liquid nitrogen.

Legal Information

3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service