SCC165
BLaER1 Human B-cell Precursor Leukemia Cell Line
BLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.
Synonym(s):
B cell Leukemia C/EBPalphaER clone 1
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
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biological source
human
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
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General description
Induction of differentiation is a therapeutic approach for cancer. Treatment of leukemias with differentiation-inducing factors in combination with pro-apoptotic compounds has been shown to be highly effective . Ectopic expression of the transcription factor C/EBPa causes reprogramming of leukemia cells, resulting in expression of a normal cell phenotype.
The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP . A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages . Cells retained their phenotype even when C/EBPa was inactivated, a characteristic of genuine cell reprogramming. C/EBPa induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.
Source:
BLaER1 cells are a single subclone isolated from C/EBPaER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 .
Biosafety Level 2:
BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.
The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP . A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages . Cells retained their phenotype even when C/EBPa was inactivated, a characteristic of genuine cell reprogramming. C/EBPa induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.
Source:
BLaER1 cells are a single subclone isolated from C/EBPaER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 .
Biosafety Level 2:
BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.
Cell Line Description
Cancer Cells
Application
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Quality
- Each vial contains ≥1×10 viable cells.
- Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
- Cells are negative for mycoplasma contamination.
- Cells tested positive for squirrel monkey retrovirus (SMRV).
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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C/EBPα Induces Highly Efficient Macrophage Transdifferentiation of B Lymphoma and Leukemia Cell Lines and Impairs Their Tumorigenicity.
Francesca Rapino et al.
Cell reports, 19(6), 1281-1281 (2017-05-13)
Grégoire Stik et al.
Nature genetics, 52(7), 655-661 (2020-06-10)
Three-dimensional organization of the genome is important for transcriptional regulation1-7. In mammals, CTCF and the cohesin complex create submegabase structures with elevated internal chromatin contact frequencies, called topologically associating domains (TADs)8-12. Although TADs can contribute to transcriptional regulation, ablation of
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