TPC-1 is a widely published and well-characterized cell line isolated from a papillary thyroid carcinoma of a female patient (6). The genome of TPC-1 contains the RET/PTC1 rearrangement found in approximately 20% of sporadic papillary carcinomas in adults (2). TPC-1 harbors a silent polymorphism in H-RAS, and is wild-type for sequences of BRAF, CNBB1, EGFR, K-RAS, RAF-1, PI3K, and TRHB (3). TPC-1 cells produce thyroglobulin (Tg) as well as expressing the thyroid differentiation marker PAX8 and the tumor progression factor podoplanin (4). In addition, TPC-1 has been utilized as an in vitro model for human cytomegalovirus latency, a potential contributor for certain human cancers (5). The TPC-1 cell line is thus an excellent system for investigating mechanisms of thyroid carcinogenesis.
Thyroid cancer is the most prevalent endocrine carcinoma, with a rapidly increasing occurrence in many countries. Human models of differentiated thyroid cancer are highly valuable for assessing the pathways and mechanisms that contribute to thyroid carcinogenesis.
1. Davies L, Welch HG (2006). Increasing incidence of thyroid cancer in the United States, 1973-2002. JAMA 295(18): 2164-2167.
2. Pilli T, et al. (2009). Potential utility and limitations of thyroid cancer cell lines as models for studying thyroid cancer. Thyroid 19(12): 1333-1342.
3. Meireles AM, et al. (2007). Molecular and genotypic characterization of human thyroid follicular cell carcinoma-derived cell lines. Thyroid 17(8): 707-715.
4. Rudzińska M, et al. (2014). The role of podoplanin in the biology of differentiated thyroid cancers. PLoS One 9(5): e96541.
5. Tanaka J, et al. (1987). Establishment and biological characterization of an in vitro human cytomegalovirus latency model. Virology 161(1): 62-72.
6. Ishizaka Y, et al. (1989). Presence of aberrant transcripts of ret proto-oncogene in a human papillary thyroid carcinoma cell line. Jpn J Cancer Res 80(12): 1149-1152.
Cell Line Description
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TPC-1 human papillary thyroid carcinoma cell line is a highly characterized model for thyroid cancer research.
• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.