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PICM01250

Millipore

Millicell® Standing Cell Culture Inserts

pore size 0.4 μm, diam. 12 mm, transparent PTFE membrane, hydrophilic, size 24 wells, sterile

Synonym(s):

Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

polystyrene housing
transparent PTFE membrane

Quality Level

400

sterility

ethylene oxide treated
sterile

feature

hydrophilic

packaging

pack of 50

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

10.5 mm

diam.

12 mm

filtration area

0.6 cm2

size

24 wells

surface area

0.6 cm2

working volume

0.6 mL

color

transparent, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Device Configuration: 24-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Biopore Membrane (hydrophilic PTFE)
- For low protein-binding, live cell viewing, and immunofluorescent applications

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Application

Research Category
Cell Culture

Packaging

Individually blister packaged

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ming Zeng et al.
Gene, 753, 144810-144810 (2020-05-30)
Meiotic entry and progression require dynamic regulation of germline gene expression. m6A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
R Le Bouffant et al.
Human reproduction (Oxford, England), 25(10), 2579-2590 (2010-07-31)
The initiation of meiosis is crucial to fertility. While extensive studies in rodents have enhanced our understanding of this process, studies in human fetal ovary are lacking. We used RT-PCR and immunohistochemistry to investigate expression of meiotic factors in human
Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
Spooner, B S, et al.
The Journal of Experimental Zoology, 269, 212-222 (1994)
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Protocols

Toluidine Blue Staining Protocol for Millicell® Culture Inserts

Toluidine blue stains the nuclear material in tissues with a high DNA and RNA content and is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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