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OP133

Sigma-Aldrich

Anti-IKKα Mouse mAb (14A231)

liquid, clone 14A231, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

14A231, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

monkey, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHUK(1147)

General description

Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
Recognizes the ~85 kDa IKKα protein in Daudi cells.
This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.

Immunogen

Human
a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag sequence

Application


Immunoblotting (1-2 g/ml, chemiluminescence)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 0.2% gelatin, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Daudi cells

Other Notes

Antibody should be titrated for optimal results in individual systems.
Zandi, E., et al. 1998 Science281, 1360.
Didonato, J.A., et al. 1997 Nature388, 548.
Lee, F.S., et al. 1997 Cell88, 213.
Mercurio, F., et al. 1997 Science278, 860.
Regnier, C.H., et al. M. 1997 Cell90, 373.
Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA94, 11758.
Verma, I.M., et al. 1995 Genes. Dev.9, 2723.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jonathan M Starkey et al.
Diabetes, 55(5), 1252-1259 (2006-04-29)
Evidence of diabetes-induced nuclear factor-kappaB (NF-kappaB) activation has been provided with DNA binding assays or nuclear localization with immunohistochemistry, but few studies have explored mechanisms involved. We examined effects of diabetes on proteins comprising NF-kappaB canonical and noncanonical activation pathways
Carlota Colomer et al.
Molecular cell, 75(4), 669-682 (2019-07-16)
Phosphorylated IKKα(p45) is a nuclear active form of the IKKα kinase that is induced by the MAP kinases BRAF and TAK1 and promotes tumor growth independent of canonical NF-κB signaling. Insights into the sources of IKKα(p45) activation and its downstream

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