NE1004

Anti-Apolipoprotein E Mouse mAb (E6D7)

liquid, clone E6D7, Calbiochem^®

• Synonym(s): Anti-ApoE
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: E6D7, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• should not react with: mouse, rabbit
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... APOE(348)

Description

General description

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.

Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.

Immunogen

Human

a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE

Application

ELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

Culture supernatant diluted in PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Brain tissue, serum

Other Notes

Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.

Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable