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MSGVS2210

Millipore

Multiscreen® 96 well Plate, hydrophilic PVDF membrane

pore size 0.22 μm, sterile

Synonym(s):

96-Well Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophilic)
flat bottom wells
polystyrene

Quality Level

description

Sterile, clear 96-well filter plate with 0.22 um pore size Hydrophilic PVDF membrane for Receptor/Ligand Binding Assays, Protein Kinase/Phosphatase Precipitation Assays & Bead-based Assays.

sterility

sterile

product line

MultiScreen®

feature

hydrophilic
lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

activity assay: suitable (protein kinase/phosphatase precipitation)
bead-based assay: suitable
ligand binding assay: suitable
receptor binding assay: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

300 μL

working volume

50-250 μL

matrix

Durapore®

pore size

0.22 μm pore size

binding type

low binding surface

shipped in

ambient

General description

MultiScreen® plates are a reliable tool for advanced drug discovery and life science research. The MultiScreen HTS 96-well filtration plates are used to filter samples and perform entire procedures, from cell growth to scintillation counting within the same plate.

Application

MultiScreen® HTS GV Filter Plate, 0.22 μm, clear, sterile has been used:
  • in high throughput solubility and redissolution screening for antibody purification via combined PEG and zinc chloride precipitation
  • to filter Prochlorococcus cultures for hydrogen peroxide measurements
  • to filter the extracted sample for spectrometry analysis

Features and Benefits

  • The HTS Filter Plates are specifically developed for high-throughput use with automated work stations.
  • The rigid sidewalls improve handling and ample surfaces are provided for bar code labels.
  • The wells are individually sealed to prevent incubation crosstalk and the underdrain is removable for access to the filters.

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Survival of Prochlorococcus in extended darkness
Coe A, et al.
Limnology and Oceanography, 61(4), 1375-1388 (2016)
Qin Gu et al.
Biotechnology progress, 36(6), e3041-e3041 (2020-06-26)
As upstream product titers increase, the downstream chromatographic capture step has become a significant "downstream bottleneck." Precipitation becomes more attractive under these conditions as the supersaturation driving force increases with the ever-increasing titer. In this study, two precipitating reagents with

Articles

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Related Content

This web page shows how the MultiScreenHTS 384-well filter plate is used to perform receptor-ligand binding assays using the Human muscarinic M1 G-protein Coupled Receptor (GPCR) as a representative model system.

This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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