MHA00P2TT

Tryptone Glucose Extract Broth

ready-to-use, with triphenyl tetrazolium chloride, suitable for microbiology, for heterotrophic bacteria

• Synonym(s): TGE broth with TTC, TGE broth with triphenyl tetrazolium chloride
• UNSPSC Code: 41106225
• eCl@ss: 32160801
• NACRES: NA.74

Properties

• sterility: sterile
• Quality Level: 100
• form: liquid
• shelf life: 12 mo.
• feature: ready-to-use
• composition: with triphenyl tetrazolium chloride
• packaging: pack of 50 tests
• technique(s): cell counting: suitable (total viable count (TVC))
• capacity: 2 mL
• filling volume: 2 mL
• color: clear medium amberslightly opalescent
• input: sample type beverage(s); sample type food(s); sample type cider; sample type bottled water; sample type beverage(s); sample type beer; sample type cosmetics; sample type wine; sample type sport drink; sample type soft drink
• pH: 7.0±0.2 (25.0 °C)
• application(s): bioburden testing; clinical; cosmetics; environmental; food and beverages; industrial qc (microbial monitoring); water monitoring; microbiology
• compatibility: for use with MF-method; for use with Microfil^®; for use with MicropreSure^®; for use with Milliflex^®
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: heterotrophic bacteria; nonselective and differential for

Description

General description

Tryptone Glucose Extract Broth with TTC is recommended for the enumeration of bacteria in water, air, milk and dairy products. It is used as a standard medium for the bacteriological plate count and is also used for the detection of thermophilic bacteria in milk and dairy products. Tryptone and yeast extract provide nitrogenous and carbonaceous compounds, long-chain amino acids, vitamin B complex and other essential growth nutrients. Glucose is the energy source, TTC is used as an indicator for easy enumeration. Colonies appear red. Red colour in the colonies is produced by reduction of triphenyl tetrazolium chloride (TTC).

Application

Tryptone Glucose Extract Broth with TTC is used as a non-selective medium to detect total heterotrophic microorganisms in water, dairy products and other liquids like Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Features and Benefits

• Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
• They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 25–35 °C

Incubation Time: 24–72 hrs

Analysis Note

Colonies appear red. Red color in the colonies is produced by reduction of triphenyl tetrazolium chloride (TTC)

QC organisms
E. coli (ATCC 25922)

Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable