MilliporeSigma
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MAC112

Sigma-Aldrich

Anti-S•Tag fusion protein Antibody

clone 26A4.1.2, from mouse

eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

26A4.1.2, monoclonal

species reactivity (predicted by homology)

all

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

shipped in

wet ice

General description

The S•Tag fusion protein is one of many polypeptide tags used to facilitate the identification and purification of many targets of interest. The gene encoding the S•Tag peptide can be added into a vector with another gene that encodes a target of interest. Such vectors, which are available through the Novagen product line, are subsequently transfected into various mammalian, bacterial, or insect cell cultures. Because the target of interest and the S•Tag peptide are expressed as one protein, identification of the target is facilitated with the use of antibodies that are specific for the respective fusion tag. Optional purification of the target may be performed using chromatography techniques that allow for the binding and removal of the respective fusion tag, if desired.

Immunogen

Linear peptide corresponding to S•Tag fusion proteins.

Application

Detect S•Tag fusion protein using this Anti-S•Tag fusion protein Antibody validated for use in Western Blotting, ICC, Immunoprecipitation.
Immunocytochemistry Analysis: A representative lot detected S•Tag fusion proteins in transfected COS-7 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitate S•Tag fusion proteins in IP.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

Evaluated by Western Blot in Posi-Tag Epitope Tag Control.

Western Blot Analysis: 0.05 µg/mL of this antibody detected S•Tag fusion proteins in 5 µL of Posi-Tag Epitope Tag Control.

Target description

~45 kDa observed. The molecular weight of S•Tag fusion protein is not 45 kDa The Posi-Tag Epitope Tag Control, which contains an S•Tag fusion protein, is observed at ~45 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Posi-Tag Epitope Tag Control

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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