MABT401
Anti-Lubricin Antibody/Proteoglycan 4, clone 9G3
clone 9G3, from mouse
Synonym(s):
Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9G3, monoclonal
species reactivity
rat, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRG4(10216)
General description
The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.
Immunogen
Recombinant protein corresponding to human Lubricin/Proteoglycan 4.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in articular cartilage tissue (Elsaid, K.A, et al. (2012). Osteoarthritis and Cartilage. 20:940-948).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in rat arterial cartilage tissue (Jay, G.D, et al. (2012). Arthritis & Rheumatism. 64(4):940-948).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in articular cartilage from rat
(Manuscript in preparation).
Western Blotting Analysis: A representative lot detected Lubricin/Proteoglycan 4 in lubricin purified from synovial fluid of patients (Manuscript in preparation).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in articular cartilage tissue (Elsaid, K.A, et al. (2012). Osteoarthritis and Cartilage. 20:940-948).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in rat arterial cartilage tissue (Jay, G.D, et al. (2012). Arthritis & Rheumatism. 64(4):940-948).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in articular cartilage from rat
(Manuscript in preparation).
Western Blotting Analysis: A representative lot detected Lubricin/Proteoglycan 4 in lubricin purified from synovial fluid of patients (Manuscript in preparation).
This Anti-Lubricin Antibody/Proteoglycan 4, clone 9G3 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.
Quality
Evaluated by Western Blotting in Lubricin purified protein.
Western Blotting Analysis: 0.02 µg/mL of this antibody detected Lubricin/Proteoglycan 4 in 1 µg of Lubricin purified protein.
Western Blotting Analysis: 0.02 µg/mL of this antibody detected Lubricin/Proteoglycan 4 in 1 µg of Lubricin purified protein.
Target description
~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Gregory D Jay et al.
Arthritis and rheumatism, 64(4), 1162-1171 (2011-12-01)
To investigate whether cartilage degeneration is prevented or minimized in a rat model of anterior cruciate ligament (ACL) injury following a single dose-escalated intraarticular injection of lubricin derived from human synoviocytes in culture. Unilateral ACL transection (ACLT) of the right
Lei Wang et al.
The Journal of surgical research, 253, 53-62 (2020-04-23)
Proteoglycan 4 (PRG4; lubricin) is a member of two gene co-expression network modules associated with human vein graft failure. However, little is known about PRG4 and the vascular system. Therefore, we have investigated the effects of recombinant human PRG4 (rhPRG4)
The impact of forced joint exercise on lubricin biosynthesis from articular cartilage following ACL transection and intra-articular lubricin's effect in exercised joints following ACL transection.
Elsaid, KA; Zhang, L; Waller, K; Tofte, J; Teeple, E; Fleming, BC; Jay, GD
Osteoarthritis and Cartilage / OARS, Osteoarthritis Research Society null
Elizabeth Feeney et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(5), 1071-1079 (2019-03-13)
The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture. Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral
Zhong Li et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(21), e2105909-e2105909 (2022-04-19)
Diseases of the knee joint such as osteoarthritis (OA) affect all joint elements. An in vitro human cell-derived microphysiological system capable of simulating intraarticular tissue crosstalk is desirable for studying etiologies/pathogenesis of joint diseases and testing potential therapeutics. Herein, a
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