MABT372

Anti-Mastl Antibody, clone 4F9

clone 4F9, from mouse

• Synonym(s): Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4F9, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001165774
• UniProt accession no.: Q96GX5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MASTL(84930)

Description

General description

Mastl is also called Serine/threonine-protein kinase greatwall (GW) and Microtubule-associated serine/threonine-protein kinase-like (MAST-L). Mastl is a serine/threonine kinase that regulates mitosis entry and maintenance during M phase through the inactivation of protein phosphatase 2A (PP2A). Mastl is also thought to be involved in megakaryocyte differentiation and the diseaseThrombocytopenia 2 (THC2).

Immunogen

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of human Mastl.

Application

Detect Mastl using this mouse monoclonal antibody, Anti-Mastl Antibody, clone 4F9 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected Mastl in human prostate cancer and human testis tissue sections.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in human oral squamos cell carcinoma lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Mastl in 10 µg of human oral squamos cell carcinoma lysate.

Target description

~97 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable