MABS430

Anti-Mcpip1, clone 2H8.2 Antibody

clone 2H8.2, from mouse

• Synonym(s): Mcpip1, Ribonuclease ZC3H12A, MCP-induced protein 1, Zinc finger CCCH domain-containing protein 12A
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H8.2, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_079355
• UniProt accession no.: Q5D1E8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ZC3H12A(80149)

Description

General description

MCP-induced protein 1 (also known as ribonuclease ZC3H12A, Zinc finger CCCH domain-containing protein 12A) is encoded by the ZC3H12A gene in human (also known as dJ423B22.1, MCPIP, MCPIP1). It belongs to the 4-member CCCH-zinc finger protein family (MCPIP1/2/3/4) that act as negative regulators in macrophage activation (PMID 18178554). MCPIP1 in particular is involved in immune response downregulation upon LPS treatment by targeting IL-6 and IL-12b with its RNase activity (PMID 22561375). MCPIP1 expresssion by MCP-1 treatment is reported to promote angiogenesis in human umbilical vein endothelial cell (HUVEC) cultures, while causing apoptotic cell death in cardiac myocytes (PMID 18364357 & 16574901).

Immunogen

GST-tagged recombinant protein corresponding to human Mcpip1.

Application

Anti-Mcpip1, clone 2H8.2 Antibody is an antibody against Mcpip1 for use in Immunohistochemistry and Immunocytochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MCPIP1 in human kidney tissue.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MCPIP1 in 10 µg of THP-1 cell lysate.

Target description

~65 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable