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antibody form
purified antibody
clone
1009
monoclonal
purified by
using protein G
species reactivity
human, rat, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
mode of action
| inhibits
General description
Recombinant fragment corresponding to 241 amino acids corresponding to six EGF repeats from human Thrombomodulin.
Specificity
Clone 1009 is a mouse monoclonal antibody that detects Thrombomodulin.
Application
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human tonsil tissue sections.
ELISA Analysis: A representative lot detected Thrombomodulin in ELISA applicaitons (Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Radioimmunoassay Analysis: A representative lot detected Thrombomodulin in Radioimmunoassay applications (Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Inhibits Activity/Function Analysis: A representative lot of this blocked thrombin binding and reduced Protein C activation (Stearns-Kurosawa, D.J., et. al. (1996). Proc Natl Acad Sci USA. 93(19):10212-6).
Flow Cytometry Analysis: A representative lot detected Thrombomodulin in Flow Cytometry applications (Stearns-Kurosawa, D.J., et. al. (1996). Proc Natl Acad Sci USA. 93(19):10212-6; Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Western Blotting Analysis: A representative lot detected Thrombomodulin in Western Blotting applications. (Xu, J., et. al. (1999). J Biol Chem. 274(10):6704-10; Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Immunocytochemistry Analysis: A representative lot detected Thrombomodulin in Immunocytochemistry applications (Campbell, J.E., et. al. (2010). Blood. 116(26):6082-91).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected Thrombomodulin in Immunohistochemistry applications (Trotman, W.E., et. al. (2011). Histochem Cell Biol. 135(2):141-52).
ELISA Analysis: A representative lot detected Thrombomodulin in ELISA applicaitons (Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Radioimmunoassay Analysis: A representative lot detected Thrombomodulin in Radioimmunoassay applications (Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Inhibits Activity/Function Analysis: A representative lot of this blocked thrombin binding and reduced Protein C activation (Stearns-Kurosawa, D.J., et. al. (1996). Proc Natl Acad Sci USA. 93(19):10212-6).
Flow Cytometry Analysis: A representative lot detected Thrombomodulin in Flow Cytometry applications (Stearns-Kurosawa, D.J., et. al. (1996). Proc Natl Acad Sci USA. 93(19):10212-6; Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Western Blotting Analysis: A representative lot detected Thrombomodulin in Western Blotting applications. (Xu, J., et. al. (1999). J Biol Chem. 274(10):6704-10; Woodley-Cook, J., et. al. (2006). Mol Cancer Ther. 5(12):3303-11).
Immunocytochemistry Analysis: A representative lot detected Thrombomodulin in Immunocytochemistry applications (Campbell, J.E., et. al. (2010). Blood. 116(26):6082-91).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected Thrombomodulin in Immunohistochemistry applications (Trotman, W.E., et. al. (2011). Histochem Cell Biol. 135(2):141-52).
Quality
Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Thrombomodulin in human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Thrombomodulin in human placenta tissue sections.
Physical properties
~60.33 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Human, Rat.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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