MABS1812M

Anti-Furin Antibody, clone 16B1.1

clone 16B1.1, from mouse

• Synonym(s): Anti-FUR, Anti-PACE, Anti-PCSK3, Anti-SPC1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 16B1.1, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001276752
• UniProt accession no.: P09958
• target post-translational modification: unmodified
• Gene Information: human ... FURIN(5045)

Description

General description

Furin (UniProt: P09958; also known as EC: 3.4.2.75, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE) is encoded by the FURIN (also known as FUR, PACE, PCSK3) gene (Gene ID: 5045) in human. Furin is an ubiquitously expressed enzyme with endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. It is single-pass type I membrane protein that shuttles between the trans-Golgi network and the cell surface. It is synthesized with a signal peptide of 26 amino acids and a propeptide region (aa 27-107) that inhibits its activity. The inhibition peptide plays the role of an intramolecular chaperone. It is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Propeptide cleavage is a prerequisite for exit of Furin molecules out of the endoplasmic reticulum. A second cleavage within the propeptide region occurs in the trans-Golgi network, followed by the release of the propeptide and the activation of Furin. Mature Furin has a luminal region (aa 108-705), a helical domain (aa 716-738), and a cytoplasmic region (aa 739-794). The cytoplasmic domain responsible for its trans-Golgi network localization and recycling from the cell surface.

Specificity

Clone 16B1.1 detects human Furin and it targets an epitope within 177 amino acids from the C-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Furin.

Application

Anti-Furin, clone 16B1.1, Cat. No. MABS1812, is a mouse monoclonal antibody that detects Furin in human cells and has been tested for use in Western Blotting.

Quality

Evaluated by Western Blotting in U-251 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Furin in 10 µg of U-251 cell lysate.

Target description

~100 kDa observed; 86.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1