MABN2728

Anti-FoxA2/HNF-3b Antibody, clone 4C7

• Synonym(s): Forkhead box A2, HNF-3 beta, FOXA2, Hepatocyte nuclear factor-3beta
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4C7, monoclonal
• mol wt: calculated mol wt 47.23 kDa; observed mol wt ~50 kDa
• purified by: using protein G
• species reactivity: human, chicken, mouse
• packaging: antibody small pack of 100
• technique(s): electron microscopy: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: NP_990101
• UniProt accession no.: Q9PWP8
• storage temp.: 2-8°C
• Gene Information: chicken ... HNF-3beta(395539)

Description

Specificity

Clone 4C7 is a mouse monoclonal antibody that detects Hepatocyte nuclear factor-3β (HNF-3β/FoxA2).

Immunogen

Recombinant Chicken Rhodopsin (1-6).

Application

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FoxA2/HNF-3b, in HepG2 cell lysate.

Tested Applications

Electrophoretic Mobility Shift Assay: A representative lot detected FoxA2/HNF-3b in Electrophoretic Mobility Shift Assay (EMSA) (Foucher, I., et al. (2003). Development. 130(9): 1867-76).

Immunofluorescence Analysis: A representative lot detected FoxA2/HNF-3b in Immunofluorescence applications (Camp, D., et al. (2014). Development. 141(20): 3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1): 132-146).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FoxA2/HNF-3b in Immunoprecipitation applications (van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).

Immunohistochemistry Applications: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Ericson, J., et al. (1996). Cell. 87(4):661-73; Kwon, O., et al. (1998). J Clin Invest. 101(10):2054-64; Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28; Camp, D., et al. (2014). Development. 141(20):3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1):132-146; Amarnath, S., et al. (2017). J Cell Sci. 130(1):119-131).

Immunocytochemistry Analysis: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Nagase, T., et al. (2009). Dev Dyn. 238(5):1118-30).

Western Blotting Analysis: A representative lot detected FoxA2/HNF-3b in Western Blotting applications (Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Hepatocyte nuclear factor-3beta (UniProt: Q9PWP8; also known as HNF-3 beta, FOXA2) is encoded by the HNF-3beta gene (Gene ID: 395539) in Chicken. FoxA2 belongs to the forkhead family of transcription factors that uses a modified helix-turn-helix motif to bind DNA as a monomer. Its DNA binding domain is localized in amino acids 151-245. It is expressed very early during development and is involved in the development of several tissues, including liver, pancreas, lung, prostate, and the nervous system. In mouse embryo, FoxA2 expression is initiated during gastrulation in the node, notochord, and floor plate. Its induction in neural plate cells is an early response to notochord-derived signals and its ectopic expression induces floor plate differentiation. In the ventral mesencephalon and cerebellum, it is co-expressed with Engrailed homeoproteins - En1 and En2, which raises the possibility that the two transcription factors interact to regulate common target genes. FoxA2 expression is observed in different regions of the developing nervous system. Along with FoxA1, it is reported to be crucial for the specification and differentiation of dopamine neurons during embryonic development. FoxA2 also functions as a suppressor of tumor metastasis by inhibition of epithelial-to-mesenchymal transition in human lung cancers. It is shown to be a key target of TGF-β1 in controlling epithelial-to-mesenchymal transition (EMT) in lung cancer cells. (Ref.: Rausa, FM., et al. (2020). Mol. Cell. Biol. 20(21); 8264-8282; Domanskyi, A., et al (2014). Front. Cell. Neurosci. 8; 275; Tang, Y., et al. (2011). Cell Res. 21(2); 316-326; Foucher, I., et al. (2003). Development 130(9); 1867-1876; Ericson, J., et al. (1996). Cell. 87(4); 661-673).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.