MABN139

Anti-mGluR5 Antibody, clone 11D9.1

clone 11D9.1, from mouse

• Synonym(s): Metabotropic glutamate receptor 5, mGluR5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11D9.1, monoclonal
• species reactivity: human, rat
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_000833
• UniProt accession no.: P41594
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GRM5(2915)

Description

General description

Metabotropic glutamate receptor 5 (mGluR5) is a member of the G-protein coupled receptor family and functions as a receptor for glutamate. mGluR5 activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.

Immunogen

GST-tagged recombinant protein corresponding to human mGluR5.

Application

Detect mGluR5 using this mouse monoclonal antibody, Anti-mGluR5 Antibody, clone 11D9.1 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected mGluR5 in normal human brain tissue.

Quality

Evaluated by Western Blotting in rat brain micropreparation tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected mGluR5 in 10 µg of rat brain micropreparation tissue lysate. The lysate was not boiled during preperation for western blotting.

Target description

~150 kDa observed. The calculated molecular weight is 130 kDa, however mGluR5 has been shown as a ~150 kDa band in western blots (Romano, C., et al. (1996). J Biol Chem. 271(45):28612-28616).

Physical form

Format: Purified

Analysis Note

Control
Rat brain micropreparation tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable