MABF935

Anti-MPR300/IGF-2R/CD222 Antibody, clone 2C2

clone 2C2, from mouse

• Synonym(s): Cation-independent mannose-6-phosphate receptor, 300 kDa mannose 6-phosphate receptor, CD222, CI Man-6-P receptor, CI-MPR, IGF-II receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, M6P/IGF2R, M6P-R
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2C2, monoclonal
• species reactivity: human
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000867
• UniProt accession no.: P11717
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... IGF2R(3482)

Description

General description

Cation-independent mannose-6-phosphate receptor (UniProt P11717; also known as 300 kDa mannose 6-phosphate receptor, CD222, CI Man-6-P receptor, CI-MPR, IGF-II receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, M6P/IGF2R, M6P-R, M6PR, MPR 300) is encoded by the IGF2R (also known as CIMPR, MPRI) gene (Gene ID 3482) in human. Mannose 6-phosphate (M6P) receptors (MPRs) mediate the delivery of newly synthesized, mannose 6-phosphate-bound lysosomal hydrolases from the secretory pathway to the endosomal system. MPRs bind lysosomal hydrolases in the trans-Golgi network (TGN) and unload the enzyme in the endosomal system. There exist a cation-independent CI-MPR (IGF2R) and a smaller CD-MPR that requires divalent cations for lysosomal hydrolases recognition. MPRs also undergo recycling between the endosomal system and the plasma membrane, where CI-MPR mediates the internalization of other ligands, including insulin-like growth factor II (IGF-II). CI-MPR/IGF2R is produced with a signal peptide (a.a. 1-40), the removal of which yields the mature receptor with a large lumenal/extracellular region (a.a. 41-2304), a transmembrane segment (a.a. 2305-2327), and a cytoplasmic domain (a.a. 2328-2491), The extracellular region is composed of 15 P-type carbohydrate recognition domains, also referred to as MRH (mannose 6-phosphate receptor homology) domains. Domains 3 and 9 bind M6P with high affinity and domain 5 binds M6P with weak affinity, while domain 11 binds IGF-II.

Specificity

Clone 2C2 targets the extracellular (lumenal) domain of IGF-II receptor (MPR). Clone 2C2 competed against IGF-II for MPR binding without affecting MPR interaction with pentamannose 6-phosphate (PMP) or beta-hexosaminidase, nor MPR-mediated beta-hexosaminidase endocytosis (Braulke, T., et al. (1988). Biochem. Res. Commun. 150(3):1287-1293; Braulke, T., et al. (1987). J. Cell Biol.;104(6):1735-1742).

Immunogen

Epitope: Extracellular (lumenal) domain.

Recombinant full-length human MPR300/IGF-2R/CD222.

Application

Affinity Binding Assay: Representative lots were labeled with 125I and employed to bind and assess IGF-II receptor (MPR) on the surface of human fibroblasts (van Rahden, V.A., et al. (2012). Hum. Mol. Genet. 21(23):5019-5038; Braulke, T., et al. (1987). J. Cell Biol.;104(6):1735-1742).
Affinity Binding Assay: A representative lot competed against IGF-II, but not pentamannose 6-phosphate (PMP), for binding immobilized IGF-II receptor (MPR) (Braulke, T., et al. (1988). Biochem. Res. Commun. 150(3):1287-1293).
Western Blotting Analysis: A representative lot was labeled with 125I and detected the ~300 kDa IGF-II receptor (MPR300) in lysates from HeLa cells and primary human macrophages (Pohl, S., et al. (2010). J. Biol. Chem. 285(31):23936-23944).
Immunohistochemistry Analysis: A representative lot detected hepatocyte membrane and cytoplasmic M6P/IGF-2R immunoreactivity in normal human liver frozen sections. A reduced immunostaining of hepatocytes restricted to the sinusoidal part of the cell membrane and an increased signals in perisinusoidal cells was seen in cirrhotic livers (Sedlaczek, N., et al. (2003). Br. J. Cancer. 88(5):733-739).
Immunocytochemistry Analysis: A representative lot immunostained late endosomes in 2% paraformaldehyde-fixed, 0.01% saponin-permeabilized human fibroblasts (Bakker, A.C., et al. (1997). J. Cell Sci. 110 (Pt 18):2227-2238).

Anti-MPR300/IGF-2R/CD222 Antibody, clone 2C2 is an antibody against MPR300/IGF-2R/CD222 for use in Immunocytochemistry, Immunohistochemistry, Affinity Binding Assay, Western Blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Vesicular Trafficking

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected MPR300/IGF-2R/CD222 in HeLa cells.

Target description

270.3 calculated. ~300 kDa reported (Pohl, S., et al. (2010). J. Biol. Chem. 285(31):23936-23944).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable