MABF1478

Anti-IFNGR1/CD119 Antibody, clone 2E2

• Synonym(s): CD119, IFN-gamma receptor 1, IFN-gamma-R-alpha, IFN-gamma-R1, Interferon gamma receptor 1, Interferon gamma receptor alpha-chain
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: hamster
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E2, monoclonal
• mol wt: calculated mol wt 52 kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100
• technique(s): flow cytometry: suitable
• isotype: IgG2
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_034641
• UniProt accession no.: P15261
• storage temp.: 2-8°C
• Gene Information: mouse ... Ifngr1(15979)

Description

Specificity

Clone 2E2 is an Armenian hamster monoclonal antibody that detects Interferon 1 receptor (IFNGR1/CD119). It targets an epitope within the extracellular domain.

Immunogen

Recombinant soluble form of -chain of mouse IFNGR1/CD119.

Application

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected IFNGR1/CD119 in one million C57BL/6 Mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected IFNGR1/CD119 in Flow Cytometry applications (Haring, J.S., et al. (2005). J Immunol. 174(11):6791-802).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Interferon gamma receptor 1 (UniProt: P15261; also known as IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, CD119) is encoded by the Ifngr1 (also known as Ifngr) gene (Gene ID: 15979) in murine species. IFNGR1/CD119 is single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 26-254), a transmembrane domain (aa 255-275), and a cytoplasmic domain (aa 276-477). It serves as a receptor subunit for interferon- that plays critical roles in antibacterial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. It associates with IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1/CD119 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, the activated JAK1 phosphorylates IFNGR1/CD119 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription. Phosphorylation of the intracellular domain of IFNGR1/CD119 also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. (Ref.: Wei, B., et al. (2010). Am. J. Transplant. 10(1); 69-80; Haring, JS., et al. (2005). J. Immunol. 174(11); 6791-6802; Qing, Y., et al. (2005). J. Biol. Chem. 280(3); 1849-1853).

Physical form

Purified armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable