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Key Documents

MABF142D

Sigma-Aldrich

Anti-Ly-6G (mouse), violetFluor® 450, clone 1A8 Antibody

clone 1A8, from rat, violetFluor® 450

Synonym(s):

Lymphocyte antigen 6G (Ly-6G), Ly-6G.1, Ly-6G

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

conjugate

violetFluor® 450

antibody form

purified antibody

antibody product type

primary antibodies

clone

1A8, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2aκ

UniProt accession no.

P35461

target post-translational modification

unmodified

Gene Information

mouse ... Ly6G(546644)

General description

The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

Application

Anti-Ly-6G (mouse), violetFluor® 450, clone 1A8 Antibody is an antibody against Ly-6G for use in Flow Cytometry.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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