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MABF111

Sigma-Aldrich

Anti-MIF Antibody, clone M1, preservative free

clone M1, from mouse

Synonym(s):

Macrophage migration inhibitory factor, MIF, Glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
neutralization: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_002406

UniProt accession no.

P14174

shipped in

wet ice

target post-translational modification

glycosylation

Gene Information

human ... MIF(4282)

General description

MIF, also known as Macrophage migration inhibitory factor, or Glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, or Phenylpyruvate tautomerase, and encoded by the gene MIF/GLIF/MMIF, is proinflammatory cytokine involved in the innate immune response to bacterial pathogens. MIF is secreted by macrophages upon stimulation by bacterial lipopolysaccharide or bacterial antigens, and the expression of MIF at sites of inflammation suggests a role as a mediator in regulating the function of macrophages in host defense. MIF also counteracts the anti-inflammatory activity of glucocorticoids and has various enzyme activities but their physiological function is not known. MIF expression is primarily lymphocytes, macrophages and immune system cells. Defects in MIF may be associated with Rheumatoid arthritis systemic juvenile disease, an inflammatory articular disorder with systemic onset beginning at age 16. Also serum levels of MIF are elevated in cases of severe sepsis and are correlated with low survival.

Immunogen

Recombinant protein corresponding to human MIF.

Application

Neutralization Assay Analysis: A representative lot from an independent laboratory detected MIF during the migration of human peripheral blood granulocytes (Rupreht, R. R., et al. (2000). Pflugers Arch. 440(5 Suppl):R78-80.).
This Anti-MIF Antibody, clone M1, preservative free is validated for use in flow cytometry, neutralizing & western blotting for the detection of MIF.

Quality

Evaluated by Flow Cytometry by Jurkat cells.

Flow Cytometry: 1 µg of this antibody detected MIF in 1X10E6 Jurkat cells.

Target description

12 kDa calculated

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ravi Kumar et al.
PloS one, 12(6), e0179012-e0179012 (2017-07-01)
Glioblastoma is the most common primary tumor of the brain and has few long-term survivors. The local and systemic immunosuppressive environment created by glioblastoma allows it to evade immunosurveillance. Myeloid-derived suppressor cells (MDSCs) are a critical component of this immunosuppression.
R R Rupreht et al.
Pflugers Archiv : European journal of physiology, 440(5 Suppl), R78-R80 (2000-09-27)
Macrophage Migration Inhibitory Factor (MIF) is a crucial component of the immune system acting together with glucocorticosteroids to regulate immunity and inflammation. Understanding of its many putative functions and action mechanisms is still ambiguous. Due to the newest findings that

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