MABE71
Anti-Histone Antibody, Pan, clone F152.C25.WJJ
clone F152C25W, from mouse
Synonym(s):
Histone H1.4, Histone H1b
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
F152C25W, monoclonal
species reactivity
human, bovine
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H2AC21(317772)
General description
This antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
Application
Anti-Histone Antibody, Pan, clone F152.C25.WJJ is a Mouse Monoclonal Antibody for detection of Histone also known as Histone H1.4, Histone H1b & has been validated in WB, ELISA.
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Mizoguchi, E., et al. (1997). Gastroenterology. 113:1828-1835.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Target description
~14-33 kDa observed
Linkage
Replaces: MAB052
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Louise D McCullough et al.
The Journal of clinical investigation, 131(17) (2021-09-29)
Inter-α inhibitor proteins (IAIPs) are a family of endogenous plasma and extracellular matrix molecules. IAIPs suppress proinflammatory cytokines, limit excess complement activation, and bind extracellular histones to form IAIP-histone complexes, leading to neutralization of histone-associated cytotoxicity in models of sepsis.
Drosophila protamine-like Mst35Ba and Mst35Bb are required for proper sperm nuclear morphology but are dispensable for male fertility.
Tirmarche, S; Kimura, S; Sapey-Triomphe, L; Sullivan, W; Landmann, F; Loppin, B
G3 (Bethesda, Md.) null
Zeynep Eren-Ghiani et al.
Cell reports, 13(11), 2327-2335 (2015-12-18)
The formation of motile spermatozoa involves the highly conserved formation of protamine-rich, tightly packed chromatin. However, genetic loss of protamine function in Drosophila and mice does not lead to significant decompaction of sperm chromatin. This indicates that other proteins act
Shuhei Kimura et al.
Open biology, 6(11) (2016-11-05)
In most animals, the bulk of sperm DNA is packaged with sperm nuclear basic proteins (SNBPs), a diverse group of highly basic chromosomal proteins notably comprising mammalian protamines. The replacement of histones with SNBPs during spermiogenesis allows sperm DNA to
Christine Dottermusch-Heidel et al.
Biology open, 3(6), 444-452 (2014-05-06)
During spermiogenesis, haploid spermatids undergo extensive chromatin remodeling events in which histones are successively replaced by more basic protamines to generate highly compacted chromatin. Here we show for the first time that H3K79 methylation is a conserved feature preceding the
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