MABE2005
Anti-RNF4 Antibody, clone 11-3.1
Synonym(s):
E3 ubiquitin-protein ligase RNF4, Protein SNURF, RING finger protein 4, Small nuclear ring finger protein
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
11-3.1, monoclonal
mol wt
calculated mol wt 21.32 kDa
observed mol wt ~32 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... RNF4(6047)
Specificity
Clone 11-3.1 is a mouse monoclonal antibody that specifically detects recombinant human RNF4 protein.
Immunogen
GST-tagged, recombinant human RNF4 protein.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
E3 ubiquitin-protein ligase RNF4 (UniProt: P78317; also known as RING finger protein 4, EC:2.3.2.27, Small nuclear ring finger protein, Protein SNURF) is encoded by the RNF4 (also known as SNURF) gene (Gene ID: 6047) in human. RNF4 gene encodes ubiquitin E3 ligase, an enzyme widely expressed in many tissues. RNF4 is mapped to human chromosome 4p16.3. The protein contains a RING-type zinc finger domain and around four small ubiquitin-related modifier (SUMO)-interaction motifs. The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates and the SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate. RNF4 is predominantly localized in the nucleus but can also be found in the cytoplasm. This dual localization allows it to function in multiple cellular compartments, dealing with SUMOylated substrates wherever they are located. The movement between these compartments can be regulated by specific signals or stress conditions. RNF4 is broadly expressed across various tissues, with notable levels in tissues that have high cellular turnover or are particularly responsive to stress, such as in immune cells and testis. It is involved in DNA repair, in transcription and chromatin regulation. It is also associated with the process of hematopoiesis. RNF4 is implicated in acute promyelocytic leukemia, acute myeloid leukemia, Fanconi anemia and aplastic anemia. Its role in maintaining protein homeostasis and responding to cellular stress makes it a key player in health and disease, with its dysfunction being linked to cancer, neurodegeneration, and developmental issues. Understanding the mechanisms regulating RNF4 activity and localization can provide insights into therapeutic strategies for these diseases. (Ref.: Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8. (PMID: 24695317).
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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