MABE1006
Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1
clone 17-3-4-1, from mouse
Synonym(s):
N6-methyladenosine, m6A
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
17-3-4-1, monoclonal
species reactivity (predicted by homology)
all
technique(s)
RIP: suitable
dot blot: suitable
isotype
IgG1λ
shipped in
wet ice
target post-translational modification
unmodified
General description
N6-Methyladenosine (m6A ) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. Fat mass and obesity-associated (FTO) and ALKBH5 are two identified demethylases that catalyze oxidative demthylation of m6A. Several functions of m6A modification have been proposed, including mRNA decay, splicing regulation, RNA nuclear export and maturation. (PMID 25171402). Cat. No. MABE1006, Anti-N6-methyladenosine (m6A), clone 17-3-4-1, is a mouse monoclonal that specifically detects RNA molecules with m6A modication.
Immunogen
Epitope: N6-methyladenosine
Hapten N6-methyladenosine-5′-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).
Application
(RNA) Immunoprecipitation Analysis: A representative lot immunoprecipitated N6-methyladenosine in IME2 transcripts (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
RNA Dot Blot Analysis: A representative lot detected N6-methyladenosine in m6A (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
RNA Dot Blot Analysis: A representative lot detected N6-methyladenosine in m6A (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1 is an antibody against N6-methyladenosine for use in Dot Blot and RNA binding protein immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Quality
Evaluated by RNA Dot Blot in oligomers containing N6-methyladenosine.
RNA Dot Blot Analysis: 1.0-2.0 μg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
RNA Dot Blot Analysis: 1.0-2.0 μg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yeast targets for mRNA methylation.
Bodi, Z; Button, JD; Grierson, D; Fray, RG
Nucleic Acids Research null
Rong-Zhang He et al.
Cancer cell international, 21(1), 616-616 (2021-11-24)
UCA1 is frequently upregulated in a variety of cancers, including CRC, and it can play an oncogenic role by various mechanisms. However, how UCA1 is regulated in cancer is largely unknown. In this study, we aimed to determine whether RNA
Kang Wei et al.
Bioengineered, 13(3), 5236-5250 (2022-02-15)
N6-methyladenosine (m6A) is one of the most significant modifications in human mRNAs. Emerging evidence indicates that m6A participates in the initiation and development of malignant tumors. Nevertheless, the biological roles and mechanism of m6A in osteosarcoma (OS) remain unclear. The
Dan Jin et al.
Journal of hematology & oncology, 12(1), 135-135 (2019-12-11)
METTL3 is an RNA methyltransferase that mediates m6A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear. The
Nate J Fry et al.
RNA (New York, N.Y.), 23(9), 1444-1455 (2017-06-15)
Post-transcriptional regulation of mRNA during oxygen deprivation, or hypoxia, can affect the survivability of cells. Hypoxia has been shown to increase stability of a subset of ischemia-related mRNAs, including VEGF. RNA binding proteins and miRNAs have been identified as important
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