MABD116
Anti-Dux4 Antibody, clone 9A12
clone 9A12, from mouse
Synonym(s):
double homeobox protein 4, Double homeobox protein 10, Double homeobox protein 4/10, Dux-4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9A12, monoclonal
species reactivity
human
technique(s)
electrophoretic mobility shift assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DUX4(100288687)
General description
Dux4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in Dux4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Ectopic expression of Dux4 in skeletal muscle activates the expression of stem cell and germline genes, and, when overexpressed in somatic cells, Dux4 can ultimately lead to cell death.
Immunogen
Epitope: domain of: C-Terminal region
recombinant protein corresponding to the domain of: C-Terminal region of Human Dux4.
Application
Detect Dux4 using this mouse monoclonal antibody, Anti-Dux4 Antibody, clone 9A12 validated for use in western blotting, ICC, Electrophoretic Mobility Shift Assay & IP.
Research Category
Stem Cell Research
Stem Cell Research
Stem Cell Research
Stem Cell Research
Research Sub Category
Muscle Physiology
Developmental Signaling
Muscle Physiology
Developmental Signaling
This antibody has been shown to work in immunocytochemistry applications (Dixit et al. 2007). This antibody has been shown to work in electrophoretic mobility shift assays (Dixit et al. 2007). This antibody has been shown to work in immunoprecipitation applications (Anseau et al., 2009).
Quality
Evaluated by Western Blot in a human skeletal muscle tissue lysate. Western Blot Analysis: A 1:250 dilution of this antibody detected Dux4 in a human skeletal muscle tissue lysate.
Target description
~58 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human skeletal muscle tissue lysate.
Human skeletal muscle tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Eugénie Ansseau et al.
PloS one, 4(10), e7482-e7482 (2009-10-16)
Facioscapulohumeral muscular dystrophy (FSHD) is a dominant disease linked to contractions of the D4Z4 repeat array in 4q35. We have previously identified a double homeobox gene (DUX4) within each D4Z4 unit that encodes a transcription factor expressed in FSHD but
Eugénie Ansseau et al.
Genes, 8(3) (2017-03-10)
FacioScapuloHumeral muscular Dystrophy (FSHD) is one of the most prevalent hereditary myopathies and is generally characterized by progressive muscle atrophy affecting the face, scapular fixators; upper arms and distal lower legs. The FSHD locus maps to a macrosatellite D4Z4 repeat
Louise A Moyle et al.
eLife, 5 (2016-11-15)
Facioscapulohumeral muscular dystrophy (FSHD) involves sporadic expression of DUX4, which inhibits myogenesis and is pro-apoptotic. To identify target genes, we over-expressed DUX4 in myoblasts and found that the receptor tyrosine kinase Ret was significantly up-regulated, suggesting a role in FSHD.
Céline Vanderplanck et al.
Skeletal muscle, 8(1), 2-2 (2018-01-14)
Facioscapulohumeral muscular dystrophy (FSHD) is associated with DNA hypomethylation at the 4q35 D4Z4 repeat array. Both the causal gene DUX4 and its homolog DUX4c are induced. DUX4c is immunodetected in every myonucleus of proliferative cells, while DUX4 is present in
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