MABC615

Anti-TNFα Receptor Antibody, clone 13F9.1

clone 13F9.1, from mouse

• Synonym(s): Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis f
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 13F9.1, monoclonal
• species reactivity: mouse, human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001056
• UniProt accession no.: P19438
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TNF(7124)

Description

General description

Tumor necrosis factor receptor superfamily member 1A (UniProt P19438; also known as CD120a, p55, p60, TNF-R1, TNF-RI, TNFR-I, Tumor necrosis factor-alpha receptor, Tumor necrosis factor receptor type 1) is encoded by the TNFRSF1A (also known as FPF, MS5, TNFAR, TNFAR55, TNFAR60, TNFR1) gene (Gene ID 7132) in human. It functions as a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. Upon activation, TNFalpha receptor mediates apoptotic, anti-viral, and other cellular signaling events. Binding of TNF-alpha to the extracellular domain of TNF receptor leads to homotrimerization. Proteolytic cleavage results in the relese of Tumor necrosis factor-binding protein 1 (TBP1). Defects in TNF receptor cause tumor necrosis factor receptor-associated periodic syndrome (TRAPS), a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia.

Specificity

Expected to react with spliced isoforms 1/FL-TNFR1, 2, 4/Delta6-TNFR1, and 5, but not isoform 3.

Immunogen

GST-tagged recombinant protein corresponding to the extracellular domain of human TNFα Receptor.

Application

Anti-TNF apha Receptor Antibody, clone 13F9.1 is a highly specific mouse monoclonal antibody, that targets TNFα Receptor & has been tested in Western Blotting, and Immunohistochemistry.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected TNFα Receptor in 10 µg of HepG2 and RAW264 cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TNFα Receptor in human colon tissue and human colon cancer cells.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TNFα Receptor in 10 µg of Jurkat cell lysate.

Target description

~50/43 kDa observed. 50.5 kDa (isoform 1/FL-TNFR1 calculated), 38.7 kDa (isoform 2 calculated), 25.6 kDa (isoform 4/Delta6-TNFR1 calculated), 24.2 kDa (isoform 5 calculated).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable