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MABC150

Sigma-Aldrich

Anti-LDH-A Antibody, clone 5D2.1

clone 5D2.1, from mouse

Synonym(s):

L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5D2.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001128711

UniProt accession no.

P00338

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LDHA(3939)

General description

L-lactate dehydrogenase A chain (LDHA), also known as Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, and encoded by the gene name LDHA/ PIG19, is a member of the LDH/MDH superfamily and LDH family. Mutations in LDHA have been assoicated with Glycogen storage disease 11 (GSD11), which is a metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

Immunogen

KLH-conjugated linear peptide corresponding to human LDH-A.

Application

Anti-LDH-A, clone 5D2.1 is an antibody against LDH-A for use in WB, IH.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LDH-A in human breast cancer sections and human kidney tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected LDH-A in 10 µg of HeLa cell lysate.

Target description

~37 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inês F Pinto et al.
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The biopharmaceutical market has been rapidly growing in recent years, creating a highly competitive arena where R&D is critical to strike a balance between clinical safety and profitability. Toward process optimization, the recent development and adoption of new process analytical

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