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MAB5264

Sigma-Aldrich

Anti-Nerve Growth Factor Receptor (p75) Antibody, clone 8211

clone 8211, Chemicon®, from mouse

Synonym(s):

NGF-Receptor p75, Low Affinity NGF Receptor, NGFR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

8211, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002507.2

UniProt accession no.

P08138

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NTF3(4908)

General description

The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.

Specificity

Reacts with NGF-receptors in human system.

Immunogen

Membrane fragments of the melanoma cell line WM-9 (Herlyn et al., 1983).

Application

Anti-Nerve Growth Factor Receptor (p75) Antibody, clone 8211 is an antibody against Nerve Growth Factor Receptor (p75) for use in IC, IH, IH(P) & WB.
Immunohistochemistry: 5-10 μg/mL

Immunocytochemistry: 5-10 μg/mL

Immunohistochemistry on paraffin sections: Cattoretti, Blood 81:1726-1738, 1993. http://www.bloodjournal.org/cgi/reprint/81/7/1726.pdf

Western Blot: 1ug/mL

Immunoabsorptive purification of NGF-receptors

Optimal working dilutions must be determined by end user.

Target description

75 kDa

Linkage

Replaces: 04-1111

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nerve growth factor receptor immunohistochemistry has a limited additional value to diagnose Hirschsprung\'s disease.
Granstrom AL, Orrego A, Svensson PJ, Almstrom M, Skikuniene J, Wester T
Pediatric Surgery International null
Production and characterization of monoclonal antibodies against human malignant melanoma.
Herlyn, M, et al.
Cancer Investigation, 1, 215-224 (1983)
Nerve growth factor is mitogenic for cancerous but not normal human breast epithelial cells.
Descamps, S, et al.
The Journal of Biological Chemistry, 273, 16659-16662 (1998)
F Hefti et al.
Annals of neurology, 20(3), 275-281 (1986-09-01)
Alzheimer's disease is associated with a pronounced loss of the cholinergic neurons that form the ascending cholinergic projections of the basal forebrain. Even though the disease is also characterized by changes in other neuronal systems and by a high frequency
Darren Carwardine et al.
PloS one, 12(12), e0188967-e0188967 (2017-12-12)
Olfactory ensheathing cell (OEC) transplantation is a promising strategy for treating spinal cord injury (SCI), as has been demonstrated in experimental SCI models and naturally occurring SCI in dogs. However, the presence of chondroitin sulphate proteoglycans within the extracellular matrix

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